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KMID : 1024520050140111071
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Journal of the Environmental Sciences
2005 Volume.14 No. 11 p.1071 ~ p.1079
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Use of comet assay as a bioassay in marine organisms exposed to genotoxicants
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Kim Gi-Beum
An Joon-Gun
Kim Jae-Won
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Abstract
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Using single cell gel electrophoresis, DNA single strand breaks were determined in various marine organisms. DNA damage on fish blood cells was detected to know whether there was a difference between Incheon, Pohang, Masan, and Tongyeong as a control site. Tongyeong showed the lowest DNA damage among the study areas. Mussels, transplanted to Masan Bay for one month, revealed high DNA damage at sites with high economical activity. In two weeks exposure of polychaete to Incheon sediments, higher DNA damage was detected in the sediment adjacent to Incheon harbor than open sea. These results suggested that the marine organism from the polluted area revealed a relatively high DNA damage. In addition, these areas might be contaminated with genotoxic compounds and comet assay was useful as a bioassay to detect DNA damage in marine organisms.
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KEYWORD
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Comet assay, Fish, Transplantation, Mussel, Polychaete, Sediment
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